Abstract
The endogenous gibberellins in rather matured seeds of Pharbitis purpurea were qualitatively analyzed by combined gas chromatography/mass spectrometry, and gibberellins A3,5,8,17,19,20,26,27,29,34, 44 and 53 were identified. A semiquantitative analysis of these gibberellins was performed on a small quantity of very young, immature seeds by gas chromatography/selected ion monitoring (GC/SIM) after purification by high performance liquid chromatography. The mass spectra of two types of these gibberellin derivatives, methyl ester-trimethylsilyl ethers and trimethylsilyl ester-trimethylsilyl ethers, were measured and their advantages and disadvantages in GC/SIM analysis are discussed.
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