Qualitative and quantitative evaluation of the comparison between the conventional and adapted spot on the law method

Abstract

The spot on the law technique is of great importance for the study of probiotic bacteria. Therefore, the main objective of this research was to develop an adaptation of this technique with the prerogative of making its application more practical and executable on a large scale in addition, validating the adaptation of this methodology based on the quantitative and qualitative analysis of the data obtained. For statistical analysis, we used the Mann-Whitney Rank Sum Test, analogous to the T Test for non-parametric data. From the results obtained, it was possible to verify that the adaptation is equivalent to the classic technique, as there was no statistical difference between the two, and that the application of the adapted methodology facilitates the execution of the spot because it presents a lower degree of difficulty.