Abstract
BackgroundTrigonella foenum-graecum (Methi) is a leguminous plant and botanically known as Trigonella foenum-graecum Linn, belong to the family Fabaceae. Trigonella foenum-graecum is used for a variety of health conditions, including digestive problems, bronchitis, tuberculosis, fevers, sore throats, wounds, arthritis, abscesses, swollen glands, skin irritations, diabetes, loss of appetite, ulcers, and menopausal symptoms, as well as in the treatment of cancer. Trigonella foenum-graecum seeds mainly contain Diosgenin [(3β,25R)-spirost-5-en3-ol], a plant-derived steroid sapogenin.ResultsThe identification and quantification results by HPTLC and HPLC studies of Trigonella foenum-graecum seeds hydrolysed Trigonella foenum-graecum seeds, coded formulation, hydrolysed coded formulation extract with standard Diosgenin biomarker showed a significant highest peak in hydrolysed Trigonella foenum-graecum seeds and hydrolysed coded formulation. The standard Diosgenin is observed in the hydrolysed form of hydrolysed Trigonella foenum-graecum seeds and hydrolysed coded formulation. The literature on Trigonella foenum-graecum confirms its activity as antidiabetic, and the peak of standard biomarker Diosgenin is seen after derivatization with anisaldehyde sulphuric agent, which possesses medicinal phytoconstituents value. Further related to future scientific aspects, more studies on its potent antidiabetic activity and multipurpose action need to be carried out with medicinal composition and its effects on the human body.ConclusionThis study aims to establish the qualitative and quantitative estimation of standard Diosgenin in reliable with coded ayurvedic formulation and Trigonella foenum-graecum seeds and its activity as antidiabetic by HPTLC and HPLC.
Highlights
Trigonella foenum-graecum (Methi) is a leguminous plant and botanically known as Trigonella foenumgraecum Linn, belong to the family Fabaceae
The aim of the present study is to develop quality control protocols for the coded ayurvedic drug by analysing the reference standard of bioactive compounds present in the formulation and respective ingredient—evaluation of High-performance thin-layer chromatography (HPTLC) fingerprint profiling along with dominant biomarkers and estimation of bioactive markers Diosgenin by High-performance liquid chromatography (HPLC)
HPTLC chromatogram gives a clear picture of standard biomarker whether it is present in ayurvedic formulations and their ingredients
Summary
Trigonella foenum-graecum (Methi) is a leguminous plant and botanically known as Trigonella foenumgraecum Linn, belong to the family Fabaceae. Trigonella foenum-graecum is used for a variety of health conditions, including digestive problems, bronchitis, tuberculosis, fevers, sore throats, wounds, arthritis, abscesses, swollen glands, skin irritations, diabetes, loss of appetite, ulcers, and menopausal symptoms, as well as in the treatment of cancer. Trigonella foenum-graecum seeds mainly contain Diosgenin [(3β,25R)-spirost-5-en3-ol], a plant-derived steroid sapogenin. India was enriched with a variety of herbs, shrubs, trees, and seeds used in the ancient Indian system of medicine and reported to possess beneficial medicinal effects in curing illness. Trigonella foenum-graecum is used in both forms in food and medicine in Asia. Seeds are eaten as sprouts and rich in secondary metabolites, potential sources of drugs, and essential oils of therapeutic importance. Due to their bitter taste, seeds are a good source of resin, protein, fibre, and mucilage. Our ayurvedic system praised this herb and documented its healing capacity in its ancient texts
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