Abstract
Qualitative and quantitative determination of four bioactive compounds including gastrodin, gastrodigenin, p-hydroxybenzaldehyde, bis(4-hydroxybenzyl)ether isolated from Gastrodia elata has been developed using high performance liquid chromatography-diode array detector coupled with electrospray ionization/mass spectrometry (HPLC-DAD-MS). The analysis of four standard compounds showed good linearity, intra- and inter-day precisions, and accuracy of them. In addition, metabolite profiling of 23 different Gastrodia elata extracts was carried out to identify their origins. Total analyzed metabolites were applied to multivariate statistical analysis including principal component analysis and hierarchical clustering analysis for pattern analysis.
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