Abstract
Tomato is an important agricultural crop world-wide. Their pigments and phenolic compounds are very important in many ways, and have been associated with health benefits such as lowering the risk of some chronic diseases. Identification of carotenoids and phenolic compounds using liquid chromatography coupled to mass spectrometry, and quantification by HPLC-DAD was carried out in the exocarp and mesocarp of the fruit in 6 different ripeness stages (mature-green, breaker, turning, pink, light red and red). Several phenolic compounds have been identified including chlorogenic acid and phenolic glycosides, such as caffeoylhexoside, coumaroyl-hexoside, feruloyl-hexoside, siringic-hexoside and rutin. Four carotenoids have been followed during ripening; β-carotene and lycopene were identified, whereas γ-carotene and lycopene-epoxide were tentatively identified, and the four of them were monitored during ripening. Fruit exocarp had higher quantities of chlorophyll, carotenoids and phenolic compounds compared to mesocarp. Chlorophylls drastically decreased, lycopene drastically increased, while β-carotene, γ-carotene and lycopene-epoxide slightly increased during fruit ripening. There were differences in the changes in phenolic compounds. Chlorogenic acid decreased and rutin increased in both the exocarp and in the mesocarp, during fruit ripening.
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