Abstract
Toosendanin (TSN) is a triterpenoid derivative found in Melia toosendan Sieb. Et Zucc (Meliaceae) or chinaberry. TSN present in the medicinal plants was first isolated and established by spectroscopic methods. In this report, high-performance liquid chromatography (HPLC) separation using columns of smaller particle size with tandem mass spectrometry (MS(n)) was used for the rapid determination of TSN in botanical extracts. A comparison of different fragmentation patterns shows that the results from positive and negative ion electrospray ionization (ESI)-MS(n) are complementary. The two modes can yield structurally significant information for the characterization and rapid identification of TSN in botanical extracts. The data obtained showed that MS(3) generated more characteristic ions that are useful for the identification of TSN in unknown samples. The separation of TSN was achieved with a water/acetonitrile gradient system using a short C18 reversed-phase column with small particle size (50 x 2.0 mm, 3.5 microm). With LC/MS, the quantitative analysis of TSN in the botanical extracts was done using external standard calibration and the method precision was found to vary from 4.3 to 7.6% (RSD, n = 5) on different days.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
