Abstract
Lianhua-Qingwen capsule (LQC) is a commonly used Chinese medical preparation to treat viral influenza and especially played a very important role in the fight against severe acute respiratory syndrome (SARS) in 2002-2003 in China. In this paper, a rapid ultraperformance liquid chromatography coupled with diode-array detector and quadrupole time-of-flight mass spectrometry (UPLC-DAD-QTOF-MS) method was established for qualitative and quantitative analysis of the major constituents of LQC. A total of 61 compounds including flavonoids, phenylpropanoids, anthraquinones, triterpenoids, iridoids, and other types of compounds were unambiguously or tentatively identified by comparing the retention times and accurate mass measurement with reference compounds or literature data. Among them, twelve representative compounds were further quantified as chemical markers in quantitative analysis, including salidroside, chlorogenic acid, forsythoside E, cryptochlorogenic acid, amygdalin, sweroside, hyperin, rutin, forsythoside A, phillyrin, rhein, and glycyrrhizic acid. The UPLC-DAD method was evaluated with linearity, limit of detection (LOD), limit of quantification (LOQ), precision, stability, repeatability, and recovery tests. The results showed that the developed quantitative method was linear, sensitive, and precise for the quality control of LQC.
Highlights
Lianhua-Qingwen capsule (LQC), developed from the two classical traditional Chinese medicine (TCM) formulae Maxing-Shigan-Tang and Yinqiao-San which have a long history of clinical application in the treatment of influenza [1], is a commonly used Chinese medical preparation to treat viral influenza and especially played an important role in the fight against severe acute respiratory syndrome (SARS) in 2002-2003 in China [2]
Some preliminary analytical methods have been developed for the quality control for LQC, including thin layer chromatography (TLC) [7], high performance liquid chromatography (HPLC) [8, 9], micellar electrokinetic capillary chromatography (MEKC) [10], and liquid chromatography tandem mass spectrometry (LC-MS/MS) [11], no systematical and comprehensive study on the chemical profiling and quality control method for LQC has been reported so far
All the results showed that the assay was satisfactory with high accuracy, good reproducibility, and high sensitivity which were beneficial to the analytical investigation and quality control for LQC
Summary
Lianhua-Qingwen capsule (LQC), developed from the two classical traditional Chinese medicine (TCM) formulae Maxing-Shigan-Tang and Yinqiao-San which have a long history of clinical application in the treatment of influenza [1], is a commonly used Chinese medical preparation to treat viral influenza and especially played an important role in the fight against severe acute respiratory syndrome (SARS) in 2002-2003 in China [2]. It is necessary to develop a rapid and sensitive method to identify and quantify the chemical constituents in LQC, which will be beneficial to investigate the effectiveness and evaluate the quality of LQC. Twelve representative compounds were quantified as chemical markers in quantitative analysis, including salidroside, chlorogenic acid, forsythoside E, cryptochlorogenic acid, amygdalin, sweroside, hyperin, rutin, forsythoside A, phillyrin, rhein, and glycyrrhizic acid. This is the first systematical and comprehensive study on the qualitative and quantitative analysis of LQC
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