Abstract
Headspace solid-phase microextraction−gas chromatography−mass spectroscopy (HS-SPME−GC−MS) has been used for both qualitative and quantitative analysis of flavor additives to tobacco. Sampling conditions for the 100 μm methyl silicone fiber, 65 μm polyacrylate fiber, 65 μm methyl silicone/divinylbenzene fiber, and 65 μm Carbowax/divinylbenzene fiber were investigated. Menthol, anethole, benzaldehyde, and tetramethylpyrazine were quantitated on spiked Kentucky Reference 1R1 tobacco. Major components in mandarin orange oil, nutmeg oil, and sweet fennel oil exhibited linear relationships with concentration of the essential oil. Limits of detection for 31 typical tobacco flavors have been determined. Keywords: Flavor additives; tobacco; solid-phase microextraction; gas chromatography; mass spectroscopy; headspace.
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