Abstract
PurposeTo perform a qualitative and quantitative analysis of catechin and quercetin in flavonoids extracted from Rosa roxburghii Tratt.MethodsTotal flavonoids were determined using ultraviolet spectrophotometry (UV) at 500 nm. The optimal gradient program started with 15 % methanol and was kept within a period of 0 – 20 min, while 25 % methanol was kept within 20 – 33 min. Subsequently, the concentration of methanol was reduced to 15 % and was held for 10 min until the next injection. Mass spectrometry spray voltage was 4,000 V, ionization temperature 350 °C, atomizer pressure 35 psi, nitrogen flow rate 8 L/min, and mass scan range 200 – 800 m/z. The detection wavelength used for catechin and quercetin was 270 and 368 nm, respectively.ResultsBased on the UV results, Rosa roxburghii Tratt content was 73.85 %, which is in agreement with the national standard. Liquid chromatography-mass spectrometry (LC-MS) results indicate that Rosa roxburghii Tratt flavonoids contained quercetin, 34.26 %, with relative standard deviation (RSD) of 2.88 % and catechin content of 2.97 % with RSD of 1.49 %.ConclusionThe proposed measurement method for determining the content of flavonoids in Rosa roxburghii Tratt has the advantage of simplicity, feasibility, good repeatability, and rapid and accurate analysis.
Highlights
Rosa roxburghii Tratt is a Rosaceae deciduous shrub of the genus Rosa, and is known as the silk flower
A total of 20 μL of the different reference solutions were injected into the drawing precision high performance liquid chromatography (HPLC) peak area as described above in order to measure catechin and quercetin
Measured according to the chromatographic conditions, the relative standard deviation (RSD) of the catechin content corresponded to 1.4%, while that of quercetin was 1.2 %
Summary
Rosa roxburghii Tratt is a Rosaceae deciduous shrub of the genus Rosa, and is known as the silk flower. Quercetin is another flavonoid of Rosa roxburghii Tratt (FRT) and has been reported to lower blood pressure, improve blood capillary resistance, reduce capillary fragility, decrease hematic fat, and increase coronary blood flow [18]. 0.4 mL of dissolved FRT sample solution was placed into a 10-mL volumetric flask; (A) was determined at 500 nm. Twenty-five milligrams of dried catechin and quercetin standard materials were weighed precisely, placed in a 25-mL volumetric flask, and dissolved in methanol at a concentration of 1 mg/mL. A total of 20 μL of the different reference solutions were injected into the drawing precision HPLC peak area as described above in order to measure catechin and quercetin.
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