Abstract
Tetrastigma hemsleyanum Diels et Gilg (T. hemsleyanums) is a kind of traditional folk medicinal plant which has been used widely in China for its antivirus, antitumor, and other clinical effects. In this study, ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry (UPLC-Q-Exactive/MS) was utilized to analyze the chemical constituents of T. hemsleyanums. Fifty-one constituents were clarified, including flavonoids, anthraquinones, esters, fatty acids, phenols, and catechins. In the subsequent quantitative analysis, the contents of ten compounds of rutin, kaempferol, astragalin, quercitrin, quercetin, vitexin-rhamnoside, isorhamnetin, vitexin, emodin-8-O-β-D-glucoside, and isoquercetin in 18 batches of T. hemsleyanums collected from different places of cultivation were determined. Meanwhile, anti-influenza virus bioactivity in vitro of the above samples was detected with Gaussia Luciferase viral titer assay. It was found that the antiviral bioactivity varied from batches to batches in accordance with content difference of the chemical constituents in T. hemsleyanums. Correlation analysis was performed with SPSS software for the association between LC-MS chemometrics and bioactivity of influenza virus inhibition, and 8 constituents of flavonoids showed positive correlation coefficient, which may provide a valuable clue for searching potential antiviral components in T. hemsleyanums.
Highlights
IntroductionTetrastigma hemsleyanum Diels et Gilg (abbreviated. as T. hemsleyanums) is a kind of medicinal and edible plant wildly growing in south China, especially in Zhejiang, Guangxi, and Yunnan provinces [1]
Tetrastigma hemsleyanum Diels et Gilg is a kind of medicinal and edible plant wildly growing in south China, especially in Zhejiang, Guangxi, and Yunnan provinces [1]
The reference standards such as kaempferol, isoquercetin, astragalin, rutin, quercetin, baicalein, and palmitic acid were purchased from Herbpurify Co., Ltd. (Chengdu, China); emodin-8-O-β-D-glucoside, quercitrin, vitexin, and vitexinrhamnoside were purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China); isorhamnetin, catechins and epicatechin were purchased from Institutes for Food and Drug control (Beijing, China)
Summary
Tetrastigma hemsleyanum Diels et Gilg (abbreviated. as T. hemsleyanums) is a kind of medicinal and edible plant wildly growing in south China, especially in Zhejiang, Guangxi, and Yunnan provinces [1]. As T. hemsleyanums) is a kind of medicinal and edible plant wildly growing in south China, especially in Zhejiang, Guangxi, and Yunnan provinces [1]. It was firstly recorded in Zhong Hua Ben Cao [2] for its effects of clearing away heat, detoxifying, removing phlegm, promoting blood circulation, and relieving pain. Nowadays, it is used in Traditional Chinese Medicine (TCM) clinics for the treatment of children with high fever (convulsions), viral meningitis, pneumonia, and hepatitis. The modern pharmacological studies had shown that it had effects of antiinflammatory, analgesic, antipyretic, antivirus, antitumor [6,7,8], immunomodulatory [9], liver protection, etc.
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