Qualitative Analysis of Some Emerging Halogenous Pollutions in Fish Sample by Comprehensive Two-Dimensional Gas Chromatography/Time-of-Flight Mass Spectrometry

Abstract

Abstract Numerous halogenated organic compounds have been identified as pollutants of concern, however, the release to the environment of many is still unrecognized. In this study, the comprehensive two dimensional gas chromatography/time of flight mass spectrometry (GC×GC-TOFMS) was applied to screen no-target halogenous pollutions in the fish sample. After extrated with hexane/actone (1:1, V/V ), cleaned-up with Gel Permeation Chromatography (GPC) and silica gel packed column, the residues were re-dissolved in the hexane and analyzed by GC×GC-TOFMS coupled with DB-5MS column and HT-8 column. In total, 72 compounds were identified as organohalogens, which including 33 polychlorinated biphenyls, 9 organochlorine pesticides, 4 polybrominated diphenyl ethers, 4 metabolites of DDT, 2 chlorinated anisole, 2 chlorostyrene, 1 thioanisole, and methyl triclosan. The rest 16 compounds were tentatively identified as halogenous pollutions, however, these compounds could not be exactly identified due to the lack of authentic standard reference mass spectrum in the library. Using external standard method, 10 dominating PCBs and 1 PBDE were quantified in 5 fish samples with S 10 PCBs and BDE-47 concentration ranged from 52-332 ng g −1 lipid and 4.8-17 ng g −1 lipid, respectively.