Abstract
In this work, a self-made microplasma jet system was used to conduct the qualitation and quantitation of inactivation with Escherichia coli as the target bacteria. The logarithmic concentration and the size of antimicrobial rings served as the evaluation parameters, respectively. The effect of various parameters on inactivation effect was studied. The results showed that the majority of bacteria had been inactivated in 30 s. The inactivation effect enhanced and then weakened with the increase of air flow rate, and receded as the extension of treatment distance. The effect with different carrier gases showed as follows: oxygen > air > nitrogen > argon. Meanwhile, the effect of different components of microplasma was studied in the optimum conditions (The flow rate was 5 L/min; inactivation distance was 2 cm). The results showed that electrically neutral active species was the main factor of inactivation rather than heating effect, ultraviolet radiation and charged particles. Finally the experiments of thallus change proved that microplasma jet had etching effect on cell membrane. It also found that microplasma could degrade organic material like protein. Furthermore, the images of scanning electron microscope (SEM) revealed the change of cell morphology step by step in the whole process of inactivation.
Highlights
With the development of society, people pay more attention to the control of biological pollution, which leads to the rapid development of disinfection technology
Standard strains of E. coli ATCC 25922 (Gram-negative bacteria) were used throughout the study to prepare specimens
Inductively coupled plasma atomic emission spectrometry (ICP-AES) was used to detect the content of K+ and Mg2+ in bacteria solution after treatment, which can characterize the permeability change of cell membrane of the treated bacterial
Summary
With the development of society, people pay more attention to the control of biological pollution, which leads to the rapid development of disinfection technology. The temperature of non-thermal plasma is similar to room temperature, and there are kinds of active species (free radicals, reactive atoms, reactive molecules, etc.) in plasma. Such kinds of active species are unable to obtain from the general chemical reactions. Those strengths are the key to the rapid development of non-thermal plasma. Microplasma jet, as a new generation of non-thermal plasma inactivation technology, has the characteristics of low power consumption, high density, and high stability, etc. The technology arouses concern of the researchers at home and abroad
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
