Abstract

A quadrupole mass spectrometer (QMS) system was constructed for the measurement of fermentation gases. This work is based on the experience in constructing QMS, respiratory and blood-gas analysers. The analyser system shown here has a microcomputer controlled QMS for multi-component analysis and a multi-channel sampling unit. The latter makes possible static and dinamic sampling of gases exhausted from the fermentor and gases dissolved in the fermentation broth. Examples are shown for its capabilities for on-line monitoring of long-term fermentation processes and off-line analysis of some components which were made volatile by adequate chemical treatments. The QMS equipment is a substantial part of a monitor system and the process control of a pilot plant of 1500l fermentors. It is capable of monitoring various fermentors quasi-simultaneously in industrial circumstances. Similar QMS technique was used for the in vivo measurement of free and dissolved gases of plants.

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