Abstract
Seed vigor is an important character of seed quality that promotes rice to germinate rapidly from soil and developing to a strong seedling, especially in the current rice direct-sowing production system. However, previous studies for seed vigor mainly concentrate in cultivars, and less reports involving in wild rice. In this study, 152 backcross inbred lines (BILs) derived from wild rice Oryza longistaminata were genotyped with re-sequencing technology, and QTLs for seed vigor related traits under normal and artificial aging treatment were analyzed. Totally, 36 QTLs were detected, of which, eight for germination potential (GP), 10 for germination rate (GR), 9 for seedling length (SL), and 9 for root length (RL). Among these, 14 novel QTLs were identified from O. longistaminata. Of which, six QTLs were related to germination, and eight related to seedling growth under aging stress. What’s more, the major QTLs q9SL1.1, q6SL1.1, and q3SL1.1 for seedling length were fallen in the same locus and fine-mapped an interval about 90 Kb. The major QTLs q9GR8.1 and q9GP8.1 related with germination were fine-mapped to an interval about 90 Kb. This work will provide us basis for breeding of high seed vigor rice in rice breeding programs and further cloning of these genes.
Highlights
As rapid development of economy, rice production is transferring from traditional getting-highyield to getting-high-efficiency by farmers
The germination potential (GP), germination rate (GR), seedling length (SL), and root length (RL) decreased about 5.00∼71.05, 5.59∼66.20, 7.22∼78.50, and 5.42∼72.89%, respectively, relative to the normal growth (Table 1). These results indicate that the seed vigor of O. longsitaminata backcross inbred lines (BILs) showed great variation among different BIL lines, and SL and RL were more sensitive than the GP and GR to the aging treatment
Out of the 36 QTLs detected in this study, four QTLs including two for germination potential, one for seedling length (q3SL5.1) and one for root length (q9RL5.1) were overlapped with those reported in previous studies
Summary
As rapid development of economy, rice production is transferring from traditional getting-highyield to getting-high-efficiency by farmers. New planting methods like direct seeding is increasingly popularizing in China dues to its lower cost and easy operation (Fujino et al, 2004). Long storage would decrease generation ability of rice grains, reduce the seedling vigor, and increase cost of rice production and seed management (Zeng et al, 2006). In southern China, the loss of rice due to seed aging or deterioration under wet and warm weather during rice mature season is becoming a serious problem. It is urgent to breed elite rice adapting to long time storage to keep high seed vigor
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
