Abstract
Fusarium head blight (FHB) is an important fungal disease affecting the yield and quality of barley and other small grains. Developing and deploying resistant barley cultivars is an essential component of an integrated strategy for reducing the adverse effects of FHB. Genetic mapping studies have revealed that resistance to FHB and the accumulation of pathogen-produced mycotoxins are controlled by many quantitative trait loci (QTL) with minor effects and are highly influenced by plant morphological traits and environmental conditions. Some prior studies aimed at mapping FHB resistance have used populations derived from crossing a Swiss landrace Chevron with elite breeding lines/cultivars. Both Chevron and Peatland, a sib-line of Chevron, were used as founders in the University of Minnesota barley breeding program. To understand the native resistance that might be present in the Minnesota breeding materials, a cross of an elite cultivar with a susceptible unadapted genotype is required. Here, a mapping population of 93 recombinant inbred lines (RILs) was developed from a cross between a moderately susceptible elite cultivar ‘Rasmusson’ and a highly susceptible Japanese landrace PI 383933. This population was evaluated for FHB severity, deoxynivalenol (DON) accumulation and various agromorphological traits. Genotyping of the population was performed with the barley iSelect 9K SNP chip and 1,394 SNPs were used to develop a genetic map. FHB severity and DON accumulation were negatively correlated with plant height (HT) and spike length (SL), and positively correlated with spike density (SD). QTL analysis using composite interval mapping (CIM) identified the largest effect QTL associated with FHB and DON on the centromeric region of chromosome 7H, which was also associated with HT, SL, and SD. A minor FHB QTL and a minor DON QTL were detected on chromosome 6H and chromosome 3H, respectively, and the Rasmusson alleles contributed to resistance. The 3H DON QTL likely represents native resistance in elite germplasm as the marker haplotype of Rasmusson at this QTL is distinct from that of Chevron. This study highlights the relationship between FHB resistance/susceptibility and morphological traits and the need for breeders to account for morphology when developing FHB resistant genotypes.
Highlights
Fusarium head blight or head scab is a major disease affecting the yield and quality of small grain cereals, especially wheat and barley worldwide
Rasmusson and Stander had the same haplotype but were different from that of Chevron, and the PI 383933 accession was different from all three. This result suggests that the 3H DON quantitative trait loci (QTL) represents native resistance in the elite germplasm in the University of Minnesota breeding program
Most of the previous mapping studies for Fusarium head blight (FHB) resistance utilized exotic sources of resistance crossed to susceptible elite cultivars or breeding lines from the Upper Midwestern United States, the region that was historically most severely impacted by
Summary
Fusarium head blight or head scab is a major disease affecting the yield and quality of small grain cereals, especially wheat and barley worldwide. Such unfavorable associations complicate introgressing FHB resistance into elite germplasm To overcome this difficulty, Massman et al (2011) conducted a genome-wide association study (GWAS) using 768 advanced barley breeding lines and identified four QTL for FHB resistance and eight QTL for DON accumulation, with each QTL explaining 1–3% of the total phenotypic variation. The aims of the present study were: (1) to identify possible novel QTL conferring resistance to FHB and DON accumulation in a representative Minnesota barley cultivar (Rasmusson) by means of a cross to a highly susceptible accession (PI 383933); and (2) to characterize the genetic architecture of disease resistance and agromorphological traits in a cross where the exotic parent is early, short and highly susceptible
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
