Abstract
The brown planthopper (Nilaparvata lugens Stål, BPH) is one of the most destructive rice pests worldwide. GXU202 is a germplasm of common wild rice (Oryza rufipogon Griff.) with high resistance to the BPH. In this study, genetic analysis indicated that the BPH-resistant phenotype of GXU202 is controlled by a major gene. Through the combination and comparison of QTL linkage and BSA-seq analyses, a novel gene locus, BPH41, conferring BPH resistance was identified. This gene locus was finely mapped to a 116-kb region delimited by W4-4–3 and W1-6–3 on chromosome 4. The markers D01031 and D01045 showed high accuracy in predicting phenotypes resistant to BPH, suggesting their reliability for marker-assisted selection of BPH41 in breeding BPH-resistant rice varieties. The present identification of BPH41 will establish a foundation for further map-based cloning and functional characterization of the gene.
Highlights
Rice (Oryza sativa L.) is one of the most important staple food crops and plays an important role in agricultural production
We focused on the identification of the gene conferring Brown planthopper (BPH) resistance in a common wild rice germplasm GXU202 through a combination of conventional QTL mapping and the bulked segregant analysis with whole-genome sequencing (BSA-seq) approach via linkage analysis
According to the identification criteria of BPH in the seedling bulk test, we evaluated the resistance potential of GXU202, 9311, and F2 populations derived from the cross GXU202/9311
Summary
Rice (Oryza sativa L.) is one of the most important staple food crops and plays an important role in agricultural production. To reduce the application of pesticide that can result in environmental pollution, high cost, pest resurgence, and stronger pathogen strain virulence(Frisvold 2019), and subsequently utilizing BPH resistance genes to develop resistant cultivars is the most promising strategy in the management of BPH(Bisht et al 2019). To this end, the exploitation of genes or genetic loci conferring BPH resistance from different germplasm resources could facilitate breeding programs for resistant rice varieties. Previous studies have indicated that the genetic basis of BPH resistance is complex, in which both major and minor resistance genes with complementary or additive effects, as well as environmental interactions are involved(Zheng et al 2020)
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