Abstract
Rice is a key crop, and metal pollution is an important factor affecting rice yield and quality. Excavating the QTLs related to the accumulation of rice grain metal content, and analyzing its candidate genes, and then improving the ability of rice to withstand metal ion stress through genetic breeding can effectively reduce the caused by metal pollution in soil. In this study, a japonica rice variety Nekken was used as the female parent, and an indica rice variety HZ was used as the male parent. After F1 generation was obtained by cross-breeding, successive selfings were performed for 120 recombinant inbred lines (RIL population) for experimental materials. Cultured under the same and suitable conditions, routine management, then we measured the metal ion content in rice grains of each line at the maturity stage. A genetic map was constructed using the RIL population to map the QTLs resistant to metal ion stress in rice. As a result, a total of 9 QTLs related to metal ion content were detected. It contains 1 multi-effect QTL related to Cu and Hg; 3 QTLs related to As; 2 QTLs related to Fe, one of which has a LOD value as high as 5.53; 2 QTLs related to Zn accumulation, The LOD of one of them is as high as 7.29. Quantitative analysis of candidate genes related to resistance to metal ion stress in these QTL intervals found that the expression levels of the four genes LOC_Os01g14440 , LOC_Os01g18584 , LOC_Os01g20160 , LOC_Os04g34600 were significantly different between parents, Combining the results of the accumulated data of parental concentrations of different metal ions, we speculated that the high expression of LOC_Os01g14440 , LOC_Os01g18584 , and LOC_Os01g20160 may greatly improve the absorption capacity and the tolerance of rice to Zn stress, while the high expression of LOC_Os04g34600 may increase the absorption capacity and the tolerance of rice to Fe stress. Through the excavation and analysis of this QTL, it was found that these genes may affect the ability of rice to withstand metal ion stress, creating conditions for further breeding and screening of rice varieties resistant to metal ion stress.
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