Abstract
AIM: To observe the effect of Qingchang Suppository (QS) on the expression of tight junction proteins zonula occluden-1 (ZO-1) and occludin in the colon of rats with trinitrobenzenesulfonic acid (TNBS)-induced ulcerative colitis (UC). METHODS: Thirty-six SD rats were randomly and equally divided into six groups: normal control group, model group, SASP group, high-, mediumand low-dose QS groups. UC was induced in rats with TNBS. The expression of tight junction proteins ZO-1 and occludin in the colon was examined by immunofluorescence. RESULTS: The immunofluorescence signals of ZO-1 and occludin were irregularly distributed and appeared disrupted in rats with UC. The average optical density of ZO-1 and occludin immunofluorescence, as calculated using image analysis software, were decreased obviously in UC rats compared to normal controls (1.802 ± 1.304 vs 3.145 ± 0.110; 0.568 ± 0.063 vs 1.538 ± 0.220, both P < 0.01). Treatment with QS upregulated the expression of ZO-1 and occludin in rats with UC (both P < 0.01). CONCLUSION: QS exerts a beneficial effect against TNBS-induced UC in rats possibly by modulating intestinal mucosal barrier and decreasing increased intestinal permeability.
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