Abstract
BackgroundQiliqiangxin (QLQX) is a preparation refined from a traditional Chinese medicine compound. It plays an important role in protecting cardiac function after myocardial infarction (MI). However, the underline mechanism of QLQX action is not clear. The purpose of this study was to detect the effects of QLQX on mitophagy after MI.MethodsMale FVB/NJ mice aged 8–10 weeks were underwent left coronary artery ligation and were orally administered either QLQX (0.25 g/kg/d) or saline. Twenty-eight days after surgical operation, the cardiac function of mice was detected by echocardiography. Electron Microscopy was used to observe the microstructure of cardiomyocytes. Myocardial apoptosis was examined by TdT-mediated dUTP Nick-End Labeling (TUNEL) and western blot. H9c2 cells were cultured in a hypoxic incubator chamber (5% CO2, 1% O2, 94% N2) for 12 h and pretreated with or without QLQX (0.5 mg/mL). The cell apoptosis, reactive oxygen species (ROS), mitochondrial membrane potential and mitophagy were detected.ResultsWhen compared to sham group, the cardiac function of MI mice decreased significantly, and their cardiomyocyte apoptosis and mitochondrial damage were more serious. These MI-induced cardiac changes could be reversed by QLQX treatment. In vitro experiments also confirmed that QLQX could protect cardiomyocytes from hypoxia-induced apoptosis and mitochondrial damage. Further study indicated that QLQX could increase the expression of Pink1 and Parkin in cardiomyocytes.ConclusionQiliqiangxin could reduce cardiomyocytes apotosis and improved heart function in infarcted heart through Pink1-mediated mitochondrial autophagy.
Highlights
Qiliqiangxin (QLQX) is a preparation refined from a traditional Chinese medicine compound
QLQX improves survival rate and cardiac function after myocardial infarction (MI) To investigate the effect of QLQX on MI, the survival rate of mice after MI operation was observed
The contractile function of the infarcted anterior wall in both saline and QLQX groups were impaired from the 2-D M-mode imaging of the left ventricle
Summary
Qiliqiangxin (QLQX) is a preparation refined from a traditional Chinese medicine compound It plays an important role in protecting cardiac function after myocardial infarction (MI). At the same time, the morbidity and mortality of left ventricular remodeling and heart failure are increasing after myocardial infarction, which has become an increasingly worrying and challenging health problem [4, 5]. Mitophagy is a kind of selective autophagy [9] It has attracted more and more attention because of its close relationship with the quantity and physiological function of mitochondria. Mitochondria provide the main energy for myocardial contraction-relaxation and control the key survival and death pathways in cells. It plays an important role in maintaining cardiac homeostasis
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