Abstract
The effects of natural and synthetic polyribonucleotides on both the polycytidylic acid-dependent polyguanylic acid polymerase (poly (G) polymerase) and Qβ replicase reactions have been investigated. Among the polymers examined, poly (U) and Qβ RNA strongly inhibited the poly (G) polymerase, but f2 RNA, E. coli tRNA, poly (A) did not. Poly (U) was found to compete with poly (C) for the enzyme, while Qβ RNA did not compete with poly (C), but reduced the Vmax, suggesting that Qβ RNA affects the poly (G) polymerase activity noncompetitively. Inhibition by Qβ RNA of the poly (G) polymerase was restored by the addition of HFI. The degree of recovery depended upon the ratio of HFI to Qβ RNA. Complete restoration of the poly (G) polymerase activity was attained at a molar ratio of 2 to 10 HFI molecules per molecule of Q/8 RNA. Poly (C) was found to inhibit the Qβ replicase activity noncompetitively. Similar but somewhat less strong inhibition occurred on the addition of poly (U), poly (A), poly (A+2U), and poly (I). Following kinetic studies, it was concluded that the poly (C) binding site was not identical with that of Qβ RNA.
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