Abstract

Serum samples from 850 patients suspected to be infected by C. burnetii were examined for the presence of antibodies against phase I and II of the microorganism. The study population was composed of people 1–70 years old, living in the rural and urban areas of the 16 prefectures in northern Greece. Characteristics of the patients were collected, including age, sex, underlying disease, occupation, contact with animals, and consumption of unpasteurised products. One serum sample was obtained at the time of admission and a second one 4 weeks later. All sera were examined using the IFA test (Focus) and the ELISA assay (Serion) according to the manufacturer’s instructions. The samples found to be positive to C. burnetii were also examined for Legionella pneumophila, Bartonella and Chlamydia species in order to exclude cross-reactivity.

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