“Pyruvate recycling” and its influence on the estimation of the pentose pathway in intact liver and Morris hepatoma 5123TC cells

Abstract

1. 1. The phenomenon of “pyruvate recycling” is demonstrated in perfused rat liver, rabbit liver in situ and in Morris Hepatoma 5123TC cells and quantitatively measured using [2- 14C]pyruvate and the method of Friedmann et al. (1971). 2. 2. Various metabolites, viz. lactate, DHAP, glucose, glucose 6-P and fructose 6-P were isolated and degraded following the metabolism of [2- 14C]pyruvate and [2- 14C]glycerol in order to assess the 14C-distributions imparted by “pyruvate recycling” reactions. The labelling of DHAP, lactate, glucose and glucose 6-P showed 14C randomizations consistent with the operation and the quantitative extent of “pyruvate recycling”. 3. 3. These findings support the proposal that the actions of “pyruvate recycling” may account for the failure to find significant levels of 14C isotope at C-1 of glucose 6-P following the metabolism of [4,5,6- 14C]-or [6- 14C]glucose by L-type pentose pathway metabolism in aerobic intact tissues. “Pyruvate recycling” diminishes the measured value of the L-type pentose cycle in intact tissues and qualifies one of the mechanistic predictions of the L-type pentose pathway which was unravelled by tracing its reactions with labelled ribose 5-P and liver enzymes (Horecker et al., 1954; Williams et al. 1978a.b) in vitro. 4. 4. The demonstration of an association of L-type pentose pathway reactions with “pyruvate recycling” by way of the common reactions of their triose-P intermediates qualifies the superficial acceptance of the predictions of the L-type pathway in vitro for the distribution of isotopic labels by aerobic tissues in vivo.