Abstract

Oxidation of cytosine and uracil was studied and the number of bacteria capable of utilizing these substrates as a single source of electrons and nitrogen was estimated. Four cultivated and two virgin soils were compared. The Warburg technique was used for measurement of oxygen consumption and the decrease of substrate concentration was determined by chromatography and spectrophotometry. Both substrates were oxidized to a greater extent in the cultivated soils. A correlation was found between the oxidation of substrates and the number of specific bacteria. Added substrates could not be detected in these soils after one mole of oxygen had been used per mole of substrate. Wir studierten die Cytosin- und Uraziloxydation und die zahlenmäßigen Veränderungen der Bakterien, die diese Substrate als alleinige Elektronenquelle auszunutzen fähig sind. Wir haben vier bearbeitete und zwei jungfräuliche Böden verglichen. Für die Messung des Sauerstoffverbrauches haben wir die Warburg-Technik angewandt. Der Substratschwund wurde mit Hilfe von Chromatographie und Spektrophotometrie ermittelt. Beide Substrate wurden stärker in den Kulturböden als in den jungfräulichen oxydiert. Zwischen Substratoxydation und Zahl der spezifischen Bakterien ergab sich eine Korrelation. Nach einem Verbrauch von 1 Mol Sauerstoff pro 1 Mol Substrat konnte dieses nicht mehr im Boden nachgewiesen werden.

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