Abstract
The alanine aminotransferase (ALAT) activities in the blood of some domestic animals have been investigated. Holoenzyme levels were measured in the absence of pyridoxal-5-phosphate (PLP) and apoenzyme levels were measured as the stimulation caused by adding PLP. In the guinea-pig, rabbit and dog, holoenzyme in blood cells is lower than in plasma. In rat, cat, pig, man, horse, goat, sheep and cow the holoenzyme is much higher in cells than plasma. The ruminant animals, goat, sheep and cow, were found to be unique in that their erythrocytes contain relatively large amounts of ALAT in the apoenzyme form. In sheep there was a positive correlation between plasma and blood cell holoenzymes and also between liver holoenzyme and blood cell apoenzyme. In experiments with sheep PLP and cortisone activated whereas DL-penicillamine and amino-oxyacetic acid inhibited ALAT activity. It is postulated that the parallel fluctuation observed in plasma and blood cell holoeuzymes is due to the effects of activators or inhibitors in blood.
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