Abstract
This paper reports the development of radiometric assays for DPN + and DPNH with assay samples of the order of 10 −12 mole. These methods utilize enzymic cycling of DPN + and DPNH by l-glutamate dehydrogenase and l-lactate dehydrogenase to convert (1- 14C)-α-ketoglutarate to l-glutamate, which is decarboxylated to 14CO 2 and counted as a measure of pyridine nucleotide concentration.
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