Pyridine nucleotides and redox-charge evolution during the induction of flowering in spinach leaves

Abstract

In the long-day plant Spinacia oleracea changes in the pool size of pyridine nucleotides have been followed under different photoperiodic conditions. In short days (vegetative state), the dark and light phases of the cycle are characterized by specific reciprocal changes in NAD and NADP pool sizes. As a consequence, the ratios of NADH/NAD+NADH and NADPH/NADP+NADPH, which are respectively considered to represent the catabolic and anabolic state of metabolism, also show a characteristic pattern. Upon transfer to continuous light, i.e. during floral induction, a decrease in anabolic metabolism is paralleled by an increase in catabolic metabolism. In the floral state, both the catabolic and the anabolic couples of the pyridine nucleotides are considerably depressed, possibly reflecting the enhanced senescence of induced leaves. The results are discussed in relation to the involvment of the nucleotides in stoichiometric coupling of metabolic compartments at the cellular level in response to environmental signals.