Pyrethroid resistance in Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae) and implications for its management in Australia

Abstract

Abstract The study was conducted to characterise the underlying resistance mechanisms responsible for high levels of pyrethroid resistance in Frankliniella occidentalis (Pergande) in Australia. Seven commercially available pyrethroids (acrinathrin, alpha‐cypermethrin, bifenthrin, deltamethrin, esfenvalerate, permethrin and tau‐fluvalinate) were evaluated against seven F. occidentalis strains collected from ornamentals, fruit and vegetables in three states of Australia. A Potter spray tower was used to test for pyrethroid resistance and all field strains were found to be resistant, with resistance ratios ranging from 15‐fold deltamethrin to 1300‐fold tau‐fluvalinate. The two most resistant strains were further tested for detoxification enzymes that could be involved in resistance. Three synergists, piperonyl butoxide (PBO), diethyl maleate (DEM) and profenofos, which, respectively, inhibit the enzymes cytochrome P‐450 monooxygenases, glutathione S‐transferases and esterases, were used. The synergism data indicate that multiple mechanisms may be involved in pyrethroid resistance in Australian populations of F. occidentalis. Among the three synergists, PBO considerably reduced pyrethroid resistance in the selected strains compared with DEM and profenofos. The practical implication for PBO use to suppress pyrethroid resistance in F. occidentalis is elaborated.