Pyrenochaeta fraxinina as colonizer of ash and sycamore petioles, its morphology, ecology, and phylogenetic connections

Abstract

Pyrenochaeta fraxinina was first described in 1913 from the state of New York (USA) on petioles of Fraxinus sp. Since then, the species has not been reported from North America and reports from the other regions of the world are very sparse. The results of this study on P. fraxinina are based on the material collected in various regions of Poland from 2012 to 2019. The material comprised 2700 previous year’s leaf petioles of Fraxinus excelsior and 1970 petioles or leaf residues of eight other deciduous tree species. As a result, the occurrence of pycnidial conidiomata of P. fraxinina was confirmed on F. excelsior (3.4% of petioles), F. mandshurica (1.5%), F. pennsylvanica (3.2%), and Acer pseudoplatanus (2.0%). The morphology of the microstructures was described based on the fresh material and compared with the holotype of P. fraxinina. The optimal temperature for the growth of the fungus in vitro was estimated as 20 °C. The analyses based on ITS-LSU rDNA sequences and a protein coding sequence of TUB2 and RPB2 genes showed that P. fraxinina isolates form a well-supported clade in the phylogenetic trees. The species proved to be closely related to Nematostoma parasiticum (asexual morph Pyrenochaeta parasitica), a species occurring on Abies alba in connection with needle browning disease. Interactions between P. fraxinina and the ash dieback pathogen, Hymenoscyphus fraxineus, were analyzed in vivo on ash petioles and in vitro in dual cultures. Among 93 petioles of F. excelsior, for which P. fraxinina conidiomata were detected, 26 were also colonized by H. fraxineus. Mostly, these two fungi occurred separately, colonizing different sections of a petiole. For all dual cultures, both fungi, P. fraxinina and H. fraxineus, showed growth inhibition toward the counterpartner. The role of P. fraxinina as a saprotrophic competitor toward H. fraxineus in ash petioles is discussed.