Pyrene drives reduced brain size during early life exposure in an estuarine fish, the red drum (Sciaenops ocellatus)

Abstract

Crude oil and the constituent polycyclic aromatic hydrocarbons (PAHs) induce a consistent suite of sub-lethal effects in early life stage fishes. It has been suggested that 3-ring PAHs drive cardiotoxicity and that all other impacts are downstream consequences of these cardiac effects. However, recent studies have documented behavioral alterations that may not be linked to cardiotoxicity. This raises the question of whether the 3-ring PAHs that drive cardiotoxicity are also responsible for the observed neurological impairments. To explore this question, we exposed embryonic red drum (Sciaenops ocellatus) – a species that exhibits greater sensitivity to craniofacial malformations than cardiotoxicity – to individual 2-ring, 3-ring, and 4-ring PAHs for 48 h after which they were assessed for sub-lethal developmental malformations. No effects were observed following exposure to naphthalene, anthracene, dibenzothiophene, phenanthrene and fluorene at doses equivalent to the ΣPAH50 effective concentration 50 for craniofacial malformation in red drum. Conversely, pyrene caused complete lethality at the original dose, and a 5× diluted dose resulted in significantly reduced brain size and spine length. Similar sub-lethal effects were also observed in chrysene at the 1× dose. These results indicate that 4-ring PAHs are driving malformations in developing red drum and suggest oil induced impairments in this species are not a downstream consequence of 3-ring PAH induced cardiac malformations.