Pyophage cocktail for the biocontrol of membrane fouling and its effect in aerobic microbial biofilm community during the treatment of antibiotics

Abstract

Membrane bioreactor systems face an inevitable challenge that is biofouling, which not only hinders the operation of the system, but also poses an environmental and medical concern caused by the increased antibiotic resistance in bacterial biofilms. This study investigates the disruption of membrane fouling using bacteriophage cocktail (Pyophage) in an aerobic membrane bioreactor for treatment of wastewater containing high non-lethal concentration of erythromycin, tetracycline and sulfamethoxazole, while also considering the effect of the cocktail on performance. The results indicate that Pyophage cocktail contributes significantly to the decrease (45%) in transmembrane pressure while also suppressing biofilm-producing bacteria compared to the control reactors. It also reconstructed biodegradation mechanism of antibiotics especially increasing the relative abundance of gram-negative bacteria by enhancement the removal rate of erythromycin and sulfamethoxazole from the aerobic system to 99%.