PVYN-Wi isolate from tomato plants in Ukraine

Abstract

PVY strains differ in their geographic distribution, pathogenicity, control strategies and the economic loss to solanaceous production. Last years, recombinant PVY strains are expanding in potato producing areas. One of the reasons is the difficult diagnostics of recombinant PVY strains. The aim of the study is to determinate the phylogenetic group (genotype) of new Ukrainian tomato isolate of potato virus Y inducing abaxial leafrolling symptoms. For the first time, the phylogenetic analysis of PVY tomato isolate ToUMP-18 (Accession No MN092711) was conducted in Ukraine. It was revealed that ToUMP-18 has the highest percentage of the 342 CP gene fragment sequence identity (98.5% nt and 98.1% aa) with European, Brazilian, Egyptian and Chinese potato isolates; with tomato isolates from Poland 08-rol, PS5, PC3, Prz1, PS4 and SL16 from Slovakia and belongs to the PVYN-Wi strain group. The Ukrainian tomato PVY isolate ToUMP-18 has 2 aa substitutions in the studied region of the CP gene in comparison with other PVY isolates that testifies about its higher divergence. This is the first report about recombinant PVYN-Wi isolate occurrence on territory of Ukraine.