Abstract
To ascertain the presence of West Nile virus (WNV), we sampled mosquitoes in 2006 in locations in southern Spain where humans had been infected. WNV genomic RNA was detected in 1 pool from unfed female Culex pipiens mosquitoes. Phylogenetic analysis demonstrated that this sequence cannot be assigned to previously described lineages of WNV.
Highlights
To ascertain the presence of West Nile virus (WNV), we sampled mosquitoes in 2006 in locations in southern Spain where humans had been infected
WNV lineage 1 was isolated from horses and birds in northern Italy, and WNV infection was described in 6 persons [3]
The phylogenetic analysis performed on a 1,813-nt fragment of the nonstructural protein 5 (NS5) gene clearly shows that the sequence recovered in Spain grouped within the branch of WNV with high values of certainty (100%)
Summary
To ascertain the presence of West Nile virus (WNV), we sampled mosquitoes in 2006 in locations in southern Spain where humans had been infected. In 2008, several countries in Europe reported WNV activity due to different lineages. The virus isolated from these birds, sparrow hawks, was WNV lineage 2 and was very homologous to 2 strains previously found in goshawks in Hungary in 2004 and 2005.
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