Purinergic signaling modulates the splenic inflammatory response in silver catfish naturally infected with Ichthyophthirius multifiliis.

Abstract

The spleen is an immune lymphatic organ linked with control of the immune response, which is important to fish health. Recent evidence has suggested the involvement of purinergic signaling in the modulation of immune and inflammatory responses through the nucleotide adenosine triphosphate (ATP) and the nucleoside adenosine (Ado), which are regulated by the enzymes nucleoside triphosphate diphosphohydrolase (NTPDase), 5'-nucleotidase, and adenosine deaminase (ADA). Thus, the aim of this study was to evaluate whether purinergic signaling can modulate the immune and inflammatory responses in the spleen of silver catfish (Rhamdia quelen) naturally infected with Ichthyophthirius multifiliis. Splenic NTPDase and 5'-nucleotidase activities increased in infected animals compared with uninfected animals, while the splenic ADA activity decreased. These data indicate that purinergic signaling can modulate the splenic immune and inflammatory responses through the regulation of ATP and Ado levels, which are known to participate in the physiological and pathological responses as pro-inflammatory and anti-inflammatory molecules, respectively. In summary, modulation of the splenic purinergic cascade has an anti-inflammatory role in reducing or preventing inflammatory damage.