Abstract
Normal pancreatic juice contains <1 mM concentrations of Ca2+, but little is known about Ca2+ homeostasis, which may be an important function of duct epithelium. Our aim was to investigate whether duct cells from human and rat pancreas express Ca2+‐transporting and Ca2+‐binding proteins and whether purinergic receptors regulate Ca2+ transport, as is known for anion transport. Expression analysis revealed that pancreatic ducts of rat and human duct cell line CFPAC‐1 (also PANC‐1 and Capan‐1) express the Na+/Ca2+ exchanger (NCX), as well as the Ca2+‐sensing receptor and the Ca2+‐binding protein calbindin 9kD. These proteins localize to the luminal and lateral membranes of pancreatic duct epithelia. Ca2+ imaging experiments showed that activation of purinergic P2X and P2Y receptors stimulated NCX in rat and human duct cells. Application of ATP to CFPAC‐1 monolayers stimulated Ca2+ transport from the luminal to the basolateral side. Taken together, these results show that pancreatic ducts express a number of Ca2+‐handling proteins and we suggest that these together with purinergic receptors may play a role in regulation of pancreatic Ca2+ transport.Support: PhD scholarship, University of Copenhagen and DNSRC 272‐05‐0420
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