Abstract

Agro-industrial sourced bioethanol fermentation produces a lipid slurry as a co-product. Corn/sorghum-based (3:2, w/w) post-fermentation lipid slurry was fractionated into crude oil and wax-rich fractions using supercritical carbon dioxide (SC-CO2). The process was optimized for temperature (35–75 °C), pressure (8–40 MPa), and time (2–6 h) for two responses (crude oil and wax yields) via response surface methodology (Box-Behnken design). The optimum conditions were determined as 35 °C, 40 MPa, and 4.1 h for maximum crude oil extraction (47.5 ± 1.4%, w/w) with minimum wax content (1.0 ± 0.2%, w/w). The crude oil fraction had a melting point of 17 °C, whereas the wax-rich fraction exhibited a melting point of 60 °C. The wax-rich fraction was composed of waxy compounds, wax esters, and aldehyde dimers. Overall, this study optimized SC-CO2 extraction conditions to selectively extract triacylglycerols from a bioethanol lipid slurry to purify waxes for food and non-food applications.

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