Abstract
Small mol. wt fractions obtained from dialysis of crude Timothy pollen extract were shown to carry immunogenic activity in monkeys. Three fractions were sequentially purified through gel filtration with Sephadex G-10 and C-M cellulose 52. Monkeys immunized with these fractions produced almost exclusively skin-sensitizing antibodies which may have been of the IgE class and differed in antigenic specificity. Analyses for sugars and amino acids in two of these fractions showed large qualitative and quantitative differences. These fractions might represent oligomeric subunits of larger antigenic molecules.
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