Abstract
Mesenchymal stem cells (MSCs) have the ability to differentiate into a variety of lineages and to renew themselves without malignant changes, and thus hold potential for many clinical applications. However, it has not been well characterized how different the properties of MSCs are depending on the tissue source in which they resided. We previously reported a novel technique for the prospective MSC isolation from bone marrow, and revealed that a combination of cell surface markers (LNGFR and THY-1) allows the isolation of highly enriched MSC populations. In this study, we isolated LNGFR+ THY-1 + MSCs from synovium using flow cytometry. The results show that the synovium tissue contained a significantly larger percentage of LNGFR + THY-1 + MSCs. We examined the colony formation and differentiation abilities of bone marrow-derived MSCs (BM-MSCs) and synovium-derived MSCs (SYN-MSCs) isolated from the same patients. Both types of MSCs exhibited a marked propensity to differentiate into specific lineages. BM-MSCs were preferentially differentiated into bone, while in the SYN-MSC culture, enhanced adipogenic and chondrogenic differentiation was observed. These data suggest that the tissue from which MSCs are isolated should be tailored according to their intended clinical therapeutic application.
Highlights
Mesenchymal stem cells (MSCs) are self-renewing cells that can differentiate into osteoblasts, chondrocytes, and adipocytes [1,2,3,4]
We show that the MSCs existed high frequency in the synovium tissue, and the pattern of surface marker expression was similar between SYN- and bone marrow (BM)- MSCs
In human BM, LNGFR and THY-1 surface makers allow the isolation of highly enriched MSCs [20]
Summary
Mesenchymal stem cells (MSCs) are self-renewing cells that can differentiate into osteoblasts, chondrocytes, and adipocytes [1,2,3,4]. These cells are found in various human tissues, including bone marrow (BM), synovium (SYN), placenta, and adipose tissue [3,5,6,7,8,9]. The characterization of MSCs isolated from those various tissues remains relatively unexplored. Because origin tissues and isolation techniques are not unified, these clinical trials led to PLOS ONE | DOI:10.1371/journal.pone.0129096. Because origin tissues and isolation techniques are not unified, these clinical trials led to PLOS ONE | DOI:10.1371/journal.pone.0129096 June 8, 2015
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
