Abstract

An isolate of blackeye cowpea mosaic virus (BlCMV) from alyce-clover (Alysicarpus vaginalis), designated as BlCMV-AC, and a Florida isolate of BlCMV (BlCMV-FL) were purified from systemically infected leaves of cowpea or white lupine. The A 260/280nm ratios obtained for purified preparations of BlCMV-AC and BlCMV-FL averaged 1.25 and 1.20, respectively. Analysis of purified BlCMV-AC by sodium dodecyl sulfate polyacrylamide gel electrophoresis revealed a major and a minor protein component with relative molecular weights of 34,500 and 31,000, respectively (.)

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