Abstract
The purification method including extraction, saponification, and separation was established for preparing free trans-astaxanthin from a high-yielding astaxanthin ester-producing strain of the microalga Haematococcus pluvialis which contained 3.67% trans-astaxanthins and 1.35% cis-astaxanthins of the dry cells. Low temperature (5°C) was chosen to minimize the degradation of astaxanthins during saponification, and 94.4% free trans-astaxanthin was obtained from trans-astaxanthin esters after 12 h of saponification. With this method, 32.2 mg trans-astaxanthin was obtained from 1 g dry algal cells. In addition, a new gradient reversed-phase HPLC method, suited for the quick analysis of free astaxanthins and astaxanthin esters in the unsaponified and saponified pigment extracts from the high-yielding astaxanthin ester-producing strain of the microalga Haematococcus pluvialis, was developed and applied to the determination of astaxanthin contents during the processes of extraction, saponification, and purification.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
