Abstract
We describe the use of a cesium chloride (CsCl) gradient as an improvement for the purification of Toxoplasma gondii oocysts from concentrated suspensions. After concentration by sucrose flotation, this technique gives a >96% recovery of very pure unsporulated or sporulated oocysts, but requires “fresh” oocysts (≤10 weeks of age). This material is suitable for biochemical and immunological analyses of environmentally resistant T. gondii oocysts.
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