Abstract
Toxic substances in Narthecium asiaticum Maxim. were isolated and purified, monitoring the oral toxicity in guinea pigs. The crude extract prepared from the methanolic extract of the plant contained 2 major saponins (C8 and C9), and 7 corresponding saponins (C1-7) on TLC. The crude extract was fractionated by a conventional method for saponin preparation. The 1-butanol fraction was rich in C8 and C9, and showed oral toxicity. C8 and C9 were isolated by fractional precipitation and silica gel column chromatography. On the basis of C-13 and H-1 nuclear magnetic resonance (NMR) spectral data, and acid and enzymatic hydrolysis of C9, C9 was confirmed to be a mixture of two furostanol saponins (C9a, C9b); a branched trisaccharide composed of galactose, glucose, and arabinose, was linked at 3 beta-C, and glucose at 26-C of sarsasapogenin and smilagenin. C8 was considered to be a furostanol saponin containing xylose instead of arabinose in the structure of C9. Oral administration of C8 and C9 caused diarrhea, proteinuria, hematuria and death in guinea pigs.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
