Abstract

The messenger RNAs for the four subunits of larval serum proteins (LSPs) 1 and 2 were purified from third instar fat body total RNA by fractionating oligo(dT)-selected RNAs on the basis of size. Translations of RNA fractions in vitro showed that the LSP mRNAs were free of other fat body messenger activities, but they could not be completely resolved from each other. The molecular weights of the LSP mRNAs, estimated from their electrophoretic mobilities in 50% formamide/1.4% agarose gels, ranged from 1.0 X 10(6) to 1.35 X 10(6). These estimates are at least 20% larger than the size of RNA necessary to encode each LSP polypeptide.

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.