Abstract
AbstractTen ion‐exchange resins were compared for their abilities to separate soybean phosphatidylcholine (PC). D113‐III resin was selected for PC purification among ten resins tested. The optimum PC adsorption conditions were: concentration of PC solution, 3 mg/mL; pH of PC solution, 7.5; and adsorption flow rate, 1 mL/min. The optimum PC desorption conditions were: eluent, 95% ethanol aqueous solution; and elution flow rate, 1 mL/min. Under these conditions, the PC content in the product and the amount of product were 94 and 80% (wt.%), respectively, and the D113‐III resin was able to be used at least six times for purifying PC. The adsorption and desorption characteristics were also studied.
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