Abstract
Sepharose-Cibacron blue (CB) F3GA for pseudo-affinity adsorption of bioproducts was synthesized and was subjected to rumen microbial enzyme evaluation through batch binding and column chromatography of xylanase. Based on equilibrium adsorption data, the best temperature, contact time, and elution agent were 30°C, 15 min, and 0.5 M NaCl (pH 6), respectively. The results show that homogenizing method had better performance in the liberation of enzyme, so that the amount of enzyme in rumen liquor approximately doubled. In pre-concentration methods, it was shown that freeze drying and precipitation of enzyme using ammonium sulfate were the best. Microbial xylanase was purified from rumen liquor to 1.85- and 11.80-fold by ammonium sulfate fractionation and Sepharose-CB column chromatography, respectively.
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