Abstract
Summary Purified horse rabies antibody has been prepared by the isoelectric fractional alcohol precipitation method of Nichol and Deutsch, with a yield of 54%. Neutralizing antibody in the final preparation is found mainly in the γ-2-G fraction. Modifications of the method of Coons were used for conjugating the purified antirabies horse antibody with fluorescein isocyanate. Staining of brain smears from rabies-infected dogs and mice with fluorescent antibody showed the diagnostic usefulness of this method in a type of communicable disease in which prompt information is most urgent.
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