Abstract
A salting out strategy is reported for purification of IgG-conjugated QD (IgG-QD) bioprobes. Adding NaCl can precipitate free IgG selectively, while the IgG-QD maintains good colloidal stability. The dynamic light scattering technique reveals that this is due to the relatively positive zeta potential of free IgG than that of the IgG-QD.
Highlights
A salting out strategy is reported for purification of Immunoglobulin G (IgG)-conjugated Quantum dots (QDs) (IgG-QD) bioprobes
The transmission electron microscopy (TEM) picture of the puri ed octylamine-gra ed poly-(acrylic acid) (OPA)-QDs displayed in Fig. 1c shows that with the assistance of phosphotungstic acid (1%), no empty OPA micelles can be observed in the NaCl-treated OPA-coated QDs (OPA-QDs), suggesting that the OPA micelles were separated from the OPA-QD solution successfully
The photoluminescence (PL) spectrum showed that the as-prepared OPA-QDs emit red uorescence at 628 nm (Fig. 1d)
Summary
A salting out strategy is reported for purification of IgG-conjugated QD (IgG-QD) bioprobes.
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