Abstract
Phytochrome was extracted from etiolated Avena seedlings and purified 1650-fold by a procedure that included isoelectric focusing in a glycerol gradient. Four phytochrome populations were separated according to their p I. The maximum of phytochrome activity was observed at pH 6.4. Only one main protein band was observed following electrophoresis in acrylamide gel of this fraction. Glycerol increases the purification yield and protects phytochrome against high pH.
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