Abstract
L-Glutamate decarboxylase (GAD), the enzyme responsible for the biosynthesis of gamma-aminobutyric acid, has been purified from rabbit brain and used for the production of a monospecific rabbit antiserum. The enzyme was purified approximately 500-fold by a combination of ion exchange, hydrophobic, hydroxyapatite, and gel filtration column chromatography, and preparative polyacrylamide gel electrophoresis. The preparation thus obtained was injected into a rabbit, producing a polyspecific antiserum. This first antiserum was used for the preparation of an immunoprecipitate containing GAD; injections of the immunoprecipitate into a second rabbit led to the production of a second, monospecific anti-GAD serum. The specificity of the antiserum for GAD has been demonstrated by enzyme precipitation, immunodiffusion, line immunoelectrophoresis, gel filtration chromatography of GAD/anti-GAD immune complexes, and immunocytochemistry in the rat cerebellum.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
