Abstract
Immunoglobulin Y (IgY) is the major protein present in the avian egg yolk. This antibody fulfils important functions in the protection of Ostrich birds against infections. The aim of this study was to demonstrate the binding capacity of Staphylococcal proteins A (SpA) to Ostrich IgY and assess purification of the IgY by SpA affinity chromatography. Chloroform polyethylene glycol (Polson), affinity chromatography, Enzyme-Linked Immunosorbent Assay (ELISA)and Western blotting methods were used in the process. Results obtained revealed that Ostrich IgY has heavy chain of 70 kDa and light chain of 30 kDa confirming results by Western blot. In addition livetins (egg yolk proteins)were shown in the protein electrophoresis that preceded the Western blot. The binding capacity between SpA and Ostrich IgY is important because SpA can be used as a reagent in immunoassays for antibody detection against microbial agents that usually infect livestocks. This is the first time the use of SpA for purification of Ostrich IgY is being reported in literature.
Highlights
Immunoglobulin Y (IgY) is the major antibody produced by birds and offers many advantages over antibodies conventionally derived from other laboratory animals [1]
This study reports on the purification of IgY from ostrich and its interaction with Staphylococcal Protein A (SpA) making this an important reagent that can be used in immunoassays for antibody detection against microbial agents usually infecting livestocks and humans
The IgY fraction was isolated by the chloroform-Polyethylene Glycol (PEG) method and further attempts of its purification was by using Protein-A affinity chromatography, where the ostrich IgY were purified from egg yolk [8]
Summary
Immunoglobulin Y (IgY) is the major antibody produced by birds and offers many advantages over antibodies conventionally derived from other laboratory animals [1]. The binding capacity between SpA and Ostrich (Struthio camelus) IgY is important because SpA can be used as a reagent in immunoassays for antibody detection against microbial agents. This study reports on the purification of IgY from ostrich and its interaction with Staphylococcal Protein A (SpA) making this an important reagent that can be used in immunoassays for antibody detection against microbial agents usually infecting livestocks and humans. This is the first time the use of SpA for purification of Ostrich IgY is being reported in literature
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