Abstract

Abstract 1. 1. Using chiefly column chromatographic methods, human renin (EC 3.4.4.15) has been purified. 2. 2. Assay has been performed using a modification of the enzyme kinetic technique used for renin estimation in body fluids. In this system, EDTA-treated human plasma has been found suitable as standard substrate for short incubations with renin solutions. 3. 3. The procedures yielded renin of increased specific activity and reduced angiotensinase activity. Using immunological criteria, the number of impurities was decreased. 4. 4. The chromatographic behaviour of human renin was the same as of pig renin.

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